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DNA Extraction
Using
prepGEM™ Storage Card Saliva
prepGEM™ Storage Card Saliva is specifically developed for extracting DNA from human saliva stored on FTA cards. The kit is adapted from the prepGEM™ saliva kit, and optimised to overcome the PCR inhibitors present in some brands of storage card. The extracted DNA is suitable for PCR applications including qPCR, STR-PCR detection and other genotyping applications.
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Workflow
Procedure
Key Data
Taq-Man real-time PCR trace of DNA extracted from saliva samples collected and stored on Whatman® FTA® Indicating Micro Cards using the prepGEM Storage Card Saliva kit.. Standards (5.5 ng/μl; 1.85 ng/μl; 0.620 ng/μl; 0.210 ng/μl; 0.068 ng/μl and 0.023 ng/μl) are shown in red. Samples (blue). DNA extractions performed on five saliva samples that had been stored on Whatman® FTA® Indicating Micro Cards. Extractions were performed on 2mm diameter punches according to the standard ZyGEM protocols as detailed in the relevant quick-start guide. Each PCR was performed in duplicate, with 5 μl of extracted DNA being used as a template in a final reaction volume of 25 μl.